Pour The mixture of lysate and Ni‐NTA agarose in to the column, and permit it to empty either by gravity flow or by implementing a vacuum to The underside on the column. A supplementary protocol will allow optional purification of protein. The purified protein is denatured and may be used https://www.facebook.com/NewTechFind/posts/pfbid032TPHc3JdtuJHT3gVQGc64WsvMnEpTWf7uC3nfkG55ojbcdzZaTURpZ191tmpeuPTl